{"id":1724,"date":"2020-03-24T11:26:56","date_gmt":"2020-03-24T10:26:56","guid":{"rendered":"https:\/\/www.purkynuvfond.cz\/?page_id=1724"},"modified":"2020-03-24T11:32:27","modified_gmt":"2020-03-24T10:32:27","slug":"rocnik-2018","status":"publish","type":"page","link":"https:\/\/www.purkynuvfond.cz\/vitezne-prace\/rocnik-2018\/","title":{"rendered":"Ro\u010dn\u00edk 2018"},"content":{"rendered":"<p><span style=\"font-size: 20px;\"><strong>V\u00edt\u011bz\u00a0Ocen\u011bn\u00ed publikace v\u00a0\u010dasopise s\u00a0nejvy\u0161\u0161\u00edm impact\u00a0factorem za rok 2018 v kategorii teoretick\u00e9 obory<\/strong><\/span><\/p>\n<p><span style=\"font-size: 20px;\"><strong>Ing. Jan Kudl\u00e1\u010dek, DiS.<\/strong><\/span><\/p>\n<p><span style=\"font-size: 20px;\"><strong>Koment\u00e1\u0159 ke \u010dl\u00e1nku\u00a0\u00a0<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/30482946\">Loss of neuronal network resilience precedes seizures and determines the ictogenic nature of interictal synaptic perturbations. <\/a><\/strong><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/30482946\">Nat Neurosci 2018 Dec;21(12):1742-1752. doi: 10.1038\/s41593-018-0278-y<\/a><\/span><br \/>\n<span style=\"font-size: 20px;\"><strong>Ztr\u00e1ta odolnosti neuron\u00e1ln\u00ed\u00a0s\u00edt\u011b p\u0159edch\u00e1z\u00ed epileptick\u00fdm z\u00e1chvat\u016fm a ur\u010duje vliv synaptick\u00fdch\u00a0perturbac\u00ed na vznik z\u00e1chvatu<\/strong><\/span><\/p>\n<p><span style=\"background-color: #ffffff;\">Epilepsie je nej\u010dast\u011bj\u0161\u00edm chronick\u00fdm neurologick\u00fdm onemocn\u011bn\u00edm, postihuj\u00edc\u00edm p\u0159ibli\u017en\u011b 1% populace ve vysp\u011bl\u00fdch zem\u00edch. Hlavn\u00edm projevem epilepsie jsou spont\u00e1nn\u00ed opakovan\u00e9 z\u00e1chvaty, kter\u00e9 p\u0159ich\u00e1z\u00ed zd\u00e1nliv\u011b n\u00e1hodn\u011b a n\u00e1hle. V na\u0161\u00ed studii jsme se zam\u011b\u0159ili na elektrografickou aktivitu mozkov\u00fdch \u0159ez\u016f udr\u017eovan\u00fdch v\u00a0<\/span>epileptogenn\u00edch<span style=\"background-color: #ffffff;\"> podm\u00ednk\u00e1ch <\/span><i>in vitro<\/i><span style=\"background-color: #ffffff;\">. Mozkov\u00e9 \u0159ezy generovaly spont\u00e1nn\u00ed z\u00e1chvatovit\u00e9 ud\u00e1losti projevuj\u00edc\u00ed se vysokou synchronizac\u00ed aktivity <\/span>neuron\u00e1ln\u00ed<span style=\"background-color: #ffffff;\"> s\u00edt\u011b.<br \/>\n<\/span>P\u0159ed ka\u017edou z\u00e1chvatovitou ud\u00e1lost\u00ed jsme pozorovali zn\u00e1mky postupn\u00e9 ztr\u00e1ty odolnosti neuron\u00e1ln\u00ed s\u00edt\u011b v\u016f\u010di synaptick\u00fdm perturbac\u00edm. Synaptick\u00e9 perturbace byly generov\u00e1ny bu\u010f mozkov\u00fdm \u0159ezem samotn\u00fdm (tzv. interikt\u00e1ln\u00ed v\u00fdboje) nebo um\u011ble vyvol\u00e1v\u00e1ny elektrickou stimulac\u00ed. Zjistili jsme, \u017ee je-li neuron\u00e1ln\u00ed s\u00ed\u0165 v\u00a0okam\u017eiku perturbace odoln\u00e1, perturbace s\u00ed\u0165 je\u0161t\u011b v\u00edce stabilizuje a oddaluje vznik z\u00e1chvatovit\u00e9 ud\u00e1losti. Je-li naopak neuron\u00e1ln\u00ed s\u00ed\u0165 n\u00e1chyln\u00e1 k\u00a0z\u00e1chvatovit\u00e9 ud\u00e1losti, perturbace dok\u00e1\u017ee takovou ud\u00e1lost naopak p\u0159ed\u010dasn\u011b spustit.<br \/>\nPozorovan\u00e1 ztr\u00e1ta stability m\u00e1 nav\u00edc charakter tzv. kritick\u00e9ho zpomalov\u00e1n\u00ed, co\u017e je velmi obecn\u00fd jev pozorovan\u00fd p\u0159ed dramatick\u00fdmi zm\u011bnami stavu r\u016fzn\u00fdch p\u0159\u00edrodn\u00edch i \u010dlov\u011bkem vytvo\u0159en\u00fdch syst\u00e9m\u016f. Kritick\u00e9 zpomalov\u00e1n\u00ed bylo nap\u0159. pozorov\u00e1no p\u0159i vym\u00edr\u00e1n\u00ed \u017eivo\u010di\u0161n\u00fdch druh\u016f, p\u0159i zm\u011bn\u00e1ch glob\u00e1ln\u00edho klimatu nebo p\u0159i vzniku hospod\u00e1\u0159sk\u00e9 krize. Uk\u00e1zali jsme, \u017ee kritick\u00e9 zpomalov\u00e1n\u00ed je pozorovateln\u00e9 t\u00e9\u017e p\u0159ed p\u0159echodem do z\u00e1chvatu, a to nejen v\u00a0mozkov\u00fdch \u0159ezech, ale tak\u00e9 u epileptick\u00fdch laboratorn\u00edch potkan\u016f a t\u00e9\u017e u n\u011bkter\u00fdch lid\u00ed s\u00a0epilepsi\u00ed.<\/p>\n<p>Tato pr\u00e1ce, kter\u00e1 vznikla v\u00a0mezin\u00e1rodn\u00edm v\u011bdeck\u00e9m kolektivu pod veden\u00edm prof. P\u0159emysla Jiru\u0161ky, Ph.D. z\u00a0Fyziologick\u00e9ho \u00fastavu AV \u010cR, m\u00e1 velk\u00fd v\u00fdznam pro pochopen\u00ed vzniku epileptick\u00fdch z\u00e1chvat\u016f a pro v\u00fdvoj nov\u00fdch l\u00e9k\u016f nebo jin\u00fdch l\u00e9\u010debn\u00fdch strategi\u00ed.<\/p>\n<p><strong>O autorovi\u00a0<\/strong><\/p>\n<p>Narodil se v roce 1988 v Praze. Vystudoval obor Multimedi\u00e1ln\u00ed technika na Fakult\u011b elektrotechnick\u00e9 \u010cVUT. Ph.D. studium v oboru v\u00fdzkumu epilepsie\u00a0 (\u010cVUT), v\u011bt\u0161inu pr\u00e1ce realizov\u00e1na na Fyziologick\u00e9m \u00fastavu AV \u010cR. V\u011bnuje se zejm\u00e9na anal\u00fdz\u00e1m EEG sign\u00e1l\u016f z anim\u00e1ln\u00edch model\u016f epilepsie nebo od pacient\u016f.<\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-size: 20px;\"><strong>V\u00edt\u011bzka Ocen\u011bn\u00ed publikace v \u010dasopise s nejvy\u0161\u0161\u00edm impact factorem\u00a0za rok 2018 v kategorii intern\u00ed obory<\/strong><\/span><\/p>\n<p><span style=\"font-size: 20px;\"><strong>Mgr. Kate\u0159ina Musilov\u00e1, Ph.D.<\/strong><\/span><\/p>\n<p><span style=\"font-size: 20px;\"><strong>Koment\u00e1\u0159 ke \u010dl\u00e1nku<\/strong>\u00a0<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/30213873\"><strong>miR-150 downregulation contributes to the high-grade transformation of follicular lymphoma by upregulating FOXP1 levels<\/strong>. Blood, 2018, 132(22): 2389-2400.<\/a><\/span><\/p>\n<p>N\u00e1\u0161 v\u00fdzkum, kter\u00fd vznikl v laborato\u0159\u00edch Intern\u00ed hematologick\u00e9 a onkologick\u00e9 kliniky Fakultn\u00ed nemocnice Brno a L\u00e9ka\u0159sk\u00e9 fakulty Masarykovy univerzity a St\u0159edoevropsk\u00e9m technologick\u00e9m institutu (CEITEC) MU, se zam\u011b\u0159il p\u0159edev\u0161\u00edm na doposud nep\u0159\u00edli\u0161 prob\u00e1danou oblast \u00falohy mal\u00fdch regula\u010dn\u00edch molekul microRNA (miRNA) v patogenezi folikul\u00e1rn\u00edho lymfomu. Folikul\u00e1rn\u00ed lymfom (FL) pat\u0159\u00ed mezi non-hodgkinsk\u00e9 B-bun\u011b\u010dn\u00e9 lymfomy, kter\u00e9 vznikaj\u00ed malign\u00ed p\u0159em\u011bnou B lymfocyt\u016f. Obecn\u011b jde o indolentn\u00ed, tedy n\u00edzce agresivn\u00ed n\u00e1dor, kter\u00fd dob\u0159e reaguje na konven\u010dn\u00ed terapii a pacienti z\u016fst\u00e1vaj\u00ed v remisi (bez projev\u016f onemocn\u011bn\u00ed) i des\u00edtky let. Nicm\u00e9n\u011b p\u0159ibli\u017en\u011b u 20 % pacient\u016f doch\u00e1z\u00ed k \u010dasn\u00e9mu relapsu (n\u00e1vratu onemocn\u011bn\u00ed) \u010di histologick\u00e9 transformaci do agresivn\u011bj\u0161\u00edho typu lymfomu, co\u017e pro pacienty p\u0159edstavuje \u010dasto fat\u00e1ln\u00ed probl\u00e9m. V\u010dasn\u00e1 identifikace a l\u00e9\u010dba takto vysoce rizikov\u00fdch pacient\u016f by mohla jejich vyhl\u00eddky zlep\u0161it. V t\u00e9to pr\u00e1ci se poprv\u00e9 poda\u0159ilo identifikovat miRNA zapojen\u00e9 do procesu transformace FL a objasnit kl\u00ed\u010dovou roli jedn\u00e9 miRNA, konkr\u00e9tn\u011b miR-150, v transformaci a obecn\u011b v agresivn\u00edm chov\u00e1n\u00ed tohoto lymfomu. Na\u0161e pr\u00e1ce uk\u00e1zala, \u017ee exprese miR-150 p\u0159i transformaci FL uniformn\u011b kles\u00e1 v souvislosti s vysok\u00fdmi hladinami onkoproteinu MYC. V d\u016fsledku toho doch\u00e1z\u00ed ke zv\u00fd\u0161en\u00ed hladin transkrip\u010dn\u00edho faktoru FOXP1, vy\u0161\u0161\u00ed proliferaci n\u00e1dorov\u00fdch bun\u011bk, zv\u00fd\u0161en\u00e9mu riziku progrese choroby a celkov\u011b zkr\u00e1cen\u00e9mu p\u0159e\u017eit\u00ed pacient\u016f. Z\u00edskan\u00e9 v\u00fdsledky v\u00fdznamn\u011b prohlubuj\u00ed poznatky o molekul\u00e1rn\u00ed podstat\u011b tohoto n\u00e1dorov\u00e9ho onemocn\u011bn\u00ed a umo\u017e\u0148uj\u00ed i potenci\u00e1ln\u00ed translaci do kliniky. D\u00edky stabilit\u011b miRNA i v b\u011b\u017en\u011b dostupn\u00e9 FFPE tk\u00e1ni (tk\u00e1\u0148 fixovan\u00e1 formalinem a zalit\u00e1 do parafinov\u00fdch blok\u016f) by tak kvantifikace exprese miR-150 mohla slou\u017eit v klinick\u00e9 praxi jako snadno detekovateln\u00fd biomarker a mohla by pomoci identifikovat pacienty s nejagresivn\u011bj\u0161\u00edm pr\u016fb\u011bhem nemoci. Nicm\u00e9n\u011b je\u0161t\u011b je pot\u0159eba prov\u00e9st d\u016fkladn\u00e9 valida\u010dn\u00ed studie a standardizovat kvantifikaci miR-150.<\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-size: 20px;\"><strong>V\u00edt\u011bzka Ocen\u011bn\u00ed publikace v \u010dasopise s nejvy\u0161\u0161\u00edm impact\u00a0factorem za rok 2018 v kategorii chirurgick\u00e9 obory<\/strong><\/span><\/p>\n<p><span style=\"font-size: 20px;\"><strong>MUDr. Barbora East, Ph.D.<\/strong><\/span><\/p>\n<p><span style=\"font-size: 20px;\"><strong>Koment\u00e1\u0159 ke \u010dl\u00e1nku<\/strong>\u00a0<strong><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29881270\">A polypropylene mesh modified with poly-\u03b5-caprolactone nanofibers in hernia repair: large animal experiment<\/a><\/strong><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29881270\">. Int J Nanomedicine. 2018 May 28;13:3129-3143. doi: 10.2147\/IJN.S159480. eCollection<\/a><\/span><\/p>\n<p>K\u00fdla v\u00a0jizv\u011b v\u00fdrazn\u011b sni\u017euje kvalitu \u017eivota a postihne zhruba ka\u017ed\u00e9ho p\u00e1t\u00e9ho pacienta po jak\u00e9koliv nitrob\u0159i\u0161n\u00ed operaci. Operace takov\u00e9 k\u00fdly se dlouho ozna\u010dovala za chirurgii nezdaru a st\u00e1le m\u00e1 velmi \u0161patn\u00e9 dlouhodob\u00e9 v\u00fdsledky. V\u00edce ne\u017e polovina pacient\u016f m\u00e1 do n\u011bkolika let po operaci recidivu. I p\u0159es velk\u00fd v\u00fdvoj v\u00a0oblasti materi\u00e1lov\u00e9ho in\u017een\u00fdrstv\u00ed se neda\u0159\u00ed tento trend zvr\u00e1tit. C\u00edlem na\u0161\u00ed pr\u00e1ce bylo vyvinout a otestovat materi\u00e1l, kter\u00fd by fungoval na biologick\u00e9m sp\u00ed\u0161e ne\u017e mechanick\u00e9m principu a otestovat ho na zv\u00ed\u0159ec\u00edm modelu. Jedn\u00e1 se o nanovl\u00e1kenn\u00fd nosi\u010d vyroben\u00fd z\u00a0biodegradabiln\u00edho polykaprolaktonu, kter\u00fd by p\u0159im\u011bl t\u011blo pacienta vytvo\u0159it regenerovat posti\u017een\u00e9 tk\u00e1n\u011b rad\u011bji ne\u017e je zhojit nefyziologickou jizvou. V\u00a0r\u00e1mci experimentu se n\u00e1m poda\u0159ilo na prase\u010d\u00edm modelu demonstrovat, \u017ee je to mo\u017en\u00e9 a \u017ee se novotvo\u0159en\u00e1 tk\u00e1\u0148 skute\u010dn\u011b chov\u00e1 p\u0159i biomechanick\u00fdch zkou\u0161k\u00e1ch bl\u00ed\u017ee zdrav\u00e9 b\u0159i\u0161n\u00ed st\u011bn\u011b ne\u017e tuh\u00e9 jizv\u011b, kter\u00e9 vznikaj\u00ed pod tradi\u010dn\u00edmi k\u00fdln\u00edmi s\u00ed\u0165kami. V\u00a0kombinaci s\u00a0takovou s\u00ed\u0165kou pak byla v\u00fdsledn\u00e1 jizva tak\u00e9 lep\u0161\u00ed mechanick\u00e9 kvality ne\u017e pod s\u00ed\u0165kou samotnou. Na z\u00e1klad\u011b tohoto experimentu se n\u00e1m poda\u0159ilo z\u00edskat grant AZV, d\u00edky kter\u00e9mu t\u00e9to materi\u00e1l d\u00e1le rozv\u00edj\u00edme.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>V\u00edt\u011bz\u00a0Ocen\u011bn\u00ed publikace v\u00a0\u010dasopise s\u00a0nejvy\u0161\u0161\u00edm impact\u00a0factorem za rok 2018 v kategorii teoretick\u00e9 obory Ing. Jan Kudl\u00e1\u010dek, DiS. Koment\u00e1\u0159 ke \u010dl\u00e1nku\u00a0\u00a0Loss of neuronal network resilience precedes seizures and determines the ictogenic nature of interictal synaptic perturbations. Nat Neurosci 2018 Dec;21(12):1742-1752. doi: 10.1038\/s41593-018-0278-y Ztr\u00e1ta odolnosti neuron\u00e1ln\u00ed\u00a0s\u00edt\u011b p\u0159edch\u00e1z\u00ed epileptick\u00fdm z\u00e1chvat\u016fm a ur\u010duje vliv synaptick\u00fdch\u00a0perturbac\u00ed na vznik z\u00e1chvatu Epilepsie je [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":49,"menu_order":21,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-1724","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.purkynuvfond.cz\/api\/wp\/v2\/pages\/1724","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.purkynuvfond.cz\/api\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.purkynuvfond.cz\/api\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.purkynuvfond.cz\/api\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.purkynuvfond.cz\/api\/wp\/v2\/comments?post=1724"}],"version-history":[{"count":3,"href":"https:\/\/www.purkynuvfond.cz\/api\/wp\/v2\/pages\/1724\/revisions"}],"predecessor-version":[{"id":1732,"href":"https:\/\/www.purkynuvfond.cz\/api\/wp\/v2\/pages\/1724\/revisions\/1732"}],"up":[{"embeddable":true,"href":"https:\/\/www.purkynuvfond.cz\/api\/wp\/v2\/pages\/49"}],"wp:attachment":[{"href":"https:\/\/www.purkynuvfond.cz\/api\/wp\/v2\/media?parent=1724"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}